rabbit polyclonal anti e2f2  (Santa Cruz Biotechnology)

Journal: Bioengineered

Article Title: MicroRNA-631 deriving from bone marrow mesenchymal stem cell exosomes facilitates the malignant behavior of non-small cell lung cancer via modulating the E2F family of transcription factor 2/phosphatidylinositol 3‐kinase/Akt signaling pathway

doi: 10.1080/21655979.2022.2036891

Figure Lengend Snippet: PCR primer sequences

Article Snippet: A total of 20 g samples were applied to 12% sulfate-polyacrylamide gel, electrophoresis was performed to separate proteins, and then electroblot was onto polyvinylidene fluoride membrane (Invitrogen, Carlsbad, CA, USA), which was sealed with 5% skimmed milk powder for 1 h. Subsequently, incubation of the membrane was with the following primary antibodies: CD9 (ab92726, Abcam), E2F2 (14,129, 1:1000, Cell Signaling Technology), CD81 (ab79559, Abcam BD Biosciences), CD63 (ab134045, Santa Cruz Biotechnology), TSG101 (ab125011, GAPDH (2118, Cell Signaling Technology) and Santa Cruz Biotechnology).

Techniques:

Journal: Bioengineered

Article Title: MicroRNA-631 deriving from bone marrow mesenchymal stem cell exosomes facilitates the malignant behavior of non-small cell lung cancer via modulating the E2F family of transcription factor 2/phosphatidylinositol 3‐kinase/Akt signaling pathway

doi: 10.1080/21655979.2022.2036891

Figure Lengend Snippet: E2F2 is the target gene of miR-631, and elevated E2F2 facilitates the malignant behavior of NSCLC.

Article Snippet: A total of 20 g samples were applied to 12% sulfate-polyacrylamide gel, electrophoresis was performed to separate proteins, and then electroblot was onto polyvinylidene fluoride membrane (Invitrogen, Carlsbad, CA, USA), which was sealed with 5% skimmed milk powder for 1 h. Subsequently, incubation of the membrane was with the following primary antibodies: CD9 (ab92726, Abcam), E2F2 (14,129, 1:1000, Cell Signaling Technology), CD81 (ab79559, Abcam BD Biosciences), CD63 (ab134045, Santa Cruz Biotechnology), TSG101 (ab125011, GAPDH (2118, Cell Signaling Technology) and Santa Cruz Biotechnology).

Techniques:

Journal: Bioengineered

Article Title: MicroRNA-631 deriving from bone marrow mesenchymal stem cell exosomes facilitates the malignant behavior of non-small cell lung cancer via modulating the E2F family of transcription factor 2/phosphatidylinositol 3‐kinase/Akt signaling pathway

doi: 10.1080/21655979.2022.2036891

Figure Lengend Snippet: MiR-631 exerts an influence on the malignant behavior of NSCLC via modulating E2F2.

Article Snippet: A total of 20 g samples were applied to 12% sulfate-polyacrylamide gel, electrophoresis was performed to separate proteins, and then electroblot was onto polyvinylidene fluoride membrane (Invitrogen, Carlsbad, CA, USA), which was sealed with 5% skimmed milk powder for 1 h. Subsequently, incubation of the membrane was with the following primary antibodies: CD9 (ab92726, Abcam), E2F2 (14,129, 1:1000, Cell Signaling Technology), CD81 (ab79559, Abcam BD Biosciences), CD63 (ab134045, Santa Cruz Biotechnology), TSG101 (ab125011, GAPDH (2118, Cell Signaling Technology) and Santa Cruz Biotechnology).

Techniques:

Journal: Bioengineered

Article Title: MicroRNA-631 deriving from bone marrow mesenchymal stem cell exosomes facilitates the malignant behavior of non-small cell lung cancer via modulating the E2F family of transcription factor 2/phosphatidylinositol 3‐kinase/Akt signaling pathway

doi: 10.1080/21655979.2022.2036891

Figure Lengend Snippet: BMSCs-Exo exerts an influence on the malignant behavior of NSCLC via the miR-631/E2F2 axis.

Article Snippet: A total of 20 g samples were applied to 12% sulfate-polyacrylamide gel, electrophoresis was performed to separate proteins, and then electroblot was onto polyvinylidene fluoride membrane (Invitrogen, Carlsbad, CA, USA), which was sealed with 5% skimmed milk powder for 1 h. Subsequently, incubation of the membrane was with the following primary antibodies: CD9 (ab92726, Abcam), E2F2 (14,129, 1:1000, Cell Signaling Technology), CD81 (ab79559, Abcam BD Biosciences), CD63 (ab134045, Santa Cruz Biotechnology), TSG101 (ab125011, GAPDH (2118, Cell Signaling Technology) and Santa Cruz Biotechnology).

Techniques:

Journal: Cell reports

Article Title: Rbf Activates the Myogenic Transcriptional Program to Promote Skeletal Muscle Differentiation

doi: 10.1016/j.celrep.2018.12.080

Figure Lengend Snippet: (A) Developing DLMs labeled using vgAME-lacZ reporter (yellow bar) and anti-Rbf antibodies from 14 to 33 h APF. Magnified images of the yellow, dashed boxes indicated on the overlay image on the left. (B) Developing DLMs at 29 h APF stained with anti-Rbf antibody, phalloidin, and DAPI in Mef2>mCherry-RNAi and Mef2>Rbf-RNAi as negative control. (C and D) Developing DLMs staged at 24 h APF (C) and 21 h APF (D) stained with phalloidin, DAPI, anti-Dp (C) and anti-E2f2 (D) antibodies. Mef2>Dp-RNAi was used as negative control. (E) Developing DLMs labeled using Mhc>tauGFP, anti-Rbf antibody, phalloidin, and DAPI from 52 to 96 h APF. Genotypes are (A) w-;vgAME-lacZ, (B) w-, UAS-Dicer2; +; Mef2-GAL4/UAS-mCherry-RNAi , and w-, UAS-Dicer2; +; Mef2-GAL4/UAS-Rbf-RNAi , (C) w-;vgAME-lacZ as WT and w-; UAS-Dp-RNAi; Mef2-GAL4, (D) y-w-, and (E) w-; P{Mhc-tauGFP}2 . Scale bars, 20 μm.

Article Snippet: 6G11,1:50, DSHB), mouse 22c10 (1:30, DSHB), rabbit anti-Mef2 (1:1,000) , mouse RBF (DX2, 1:20, , rabbit polyclonal anti-Dp (#212, 1:300) , rabbit anti-E2f2 antibodies (#79), rabbit anti-Cleaved Drosophila Dcp-1 (Asp216, 1:500, Cell Signaling), rat anti-Kettin (MAC155,1:1000, Babraham Institute) and rabbit anti-pH3 (Millipore, 1:200), Rhodamine–Phalloidin or fluorescein isothiocyanate–Phalloidin were used to counterstain and stain thin filaments, and 4,6-diamidino-2-phenylindole (DAPI) for nucleus staining.

Techniques: Labeling, Staining, Negative Control